Stem Cell Transcriptional Networks Methods and Protocols

<p>This second edition provides techniques used to study of the underlying transcriptional programs of stem cells that promote self-renewal and differentiation. Chapters detail next-generation sequencing technologies, data analysis, protocols on analysis and visualization of single-cell RNA-Seq data, analysis of 3D chromatin architecture, interpretation of large-scale interaction networks, transcriptional networks in embryonic and adult stem cells, derivation of stem cells, and transcriptional programs that promote reprogramming, transdifferentiation, and cancer formation. Written in the highly successful <i>Methods in Molecular Biology </i>series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.</p> <p> Authoritative and cutting-edge, <i>Stem Cell Transcriptional Network: Methods and Protocols, Second Edition </i>aims to provide a key resource for biologists seeking to interrogate these vital networks.</p>